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The protestor ran onto the court between sets during the match between Stefanos Tsitsipas and Diego Schwartzman.
Williams recently alluded to Brady's "trend" of retiring then unretiring in interviews on "The Tonight Show" and "Good Morning America."
Carlos Alcaraz fulfilled his destiny with an entertaining and emphatic defeat of Casper Ruud.
Serena and Venus have done for tennis what Tiger Woods was supposed to do for golf, the U.S.'s other country club sport.
Nick Kyrgios punished multiple tennis rackets after being bounced out of the US Open quarterfinals by Karen Khachanov,
Women's No. 1 Iga Swiatek needed a rally to win, and Rafael Nadal has now lost his first match at a grand slam this year.
Follow along with Yahoo Sports as we recap the biggest moments from the seventh day of the U.S. Open.
Serena Williams lost in the third round of the US Open on Friday night in the final match of her career.
Ajla Tomljanovic beat Serena Williams in a thrilling three-hour battle on Friday night at the US Open.
They changed the sport and inspired lives. They did it in lockstep. If there were rivalries, they were hidden. If there was jealousy, it stayed a secret. The mutual support has been inspiring.
Serena has always loved herself out loud, her gorgeous curves and luscious lips and coiled hair, and that has resonated with Black women, who for so long have been told that those things were unappealing.